The Heir's Scandalous Affair by Jennifer Lewis

Competition between the analyte and ligand-GFP conjugate for binding sites on the anti-ligand permits an assay for an unknown amount of the analyte. The anti-ligand also has a specific binding affinity for the analyte. A ligand-GFP conjugate has a specific binding affinity for an anti-ligand immobilized on a support. The resulting conjugates retain the fluorescence characteristics of the unmodified protein and are used along with avidin-coated magnetic beads in the development of the assay.Ī heterogeneous binding assay for an analyte in a fluid sample is developed, which uses a green fluorescent protein (GFP) label.

The mutant of GFP that contains a six-histidine tail to facilitate purification on an immobilized metal affinity column is chemically modified to incorporate biotin moieties. Picomolar amounts of analyte can be detected. A preferred assay employing an antibody and its antigen is illustrated for a fusion protein containing GFP and an antigenic determinant.

Preferred specific binding pairs for use in the assay are biotin:avidin, and a selected antibody and its antigen. (303) 233-0942, (303) 353-4173, (303) 832-1658, (720) 242-7972ġ03 Cloverhill Ct, Locust Grove, VA 22508ġ004 County Route 124, Rexville, NY 14877ġ015 E 18Th Ave Apt 115, Ellensburg, WA 98926ġ040 Eagle Bluff Ct, Greensboro, GA 30642ġ045 N Utah St Apt 508, Arlington, VA 22201Ģ6861 Via Zaragosa, San Juan Capistrano, CA 92691Ģ6370 Main Street Ext, Crisfield, MD 21817ĬOUNCIL OF FILIPINO ORGANIZATIONS OF ARIZONAĭirector 4519 W Wilshire Dr, Phoenix, AZ 85035ġ02 Marshside Dr, Saint Augustine, FL 32080ģ000 N Prairie Ave, Saint Louis, MO 63107Ĩ38 Evergreen Point Road, Medina, WA 98039ģ235 Fairfield Ave S, Saint Petersburg, FL 33712Ģ193 Regents Circle, West Palm Beach, FL 33409ģ29 S Bromeliad, West Palm Beach, FL 33401Ī heterogeneous binding assay for an analyte in a fluid sample is developed, which uses a green fluorescent protein (GFP) label.

The Heir's Scandalous Affair by Jennifer Lewis